Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Breast
Cell type
SUM 44PE
Primary Tissue
Breast
Tissue Diagnosis
Unknown

Attributes by original data submitter

Sample

source_name
SUM44-WM-E2-GATA3
cell line
SUM44PE breast carcinoma cell line
agent
Estrogen
chip antibody
GATA3 (D13C9, Cell Signaling Technology )

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted with a sarkosyl based buffer and sonicated to a size range of 200-300 bp DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Takara Bio USA)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
49658816
Reads aligned (%)
92.9
Duplicates removed (%)
58.9
Number of peaks
1866 (qval < 1E-05)

hg19

Number of total reads
49658816
Reads aligned (%)
92.0
Duplicates removed (%)
60.8
Number of peaks
2058 (qval < 1E-05)

Base call quality data from DBCLS SRA